Publication

2020

Asymmetric distribution of TLR3 leads to a polarized immune response in human intestinal epithelial cells. Stanifer ML, Mukenhirn M, Muenchau S, Pervolaraki K, Kanaya T, Albrecht D, Odendall C, Hielscher T, Haucke V, Kagan JC, Bartfeld S, Ohno H, Boulant S. Nat Microbiol.

 

Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells. Stanifer ML, Kee C, Cortese M, Zumaran CM, Triana S, Mukenhirn M, Kraeusslich HG, Alexandrov T, Bartenschlager R, Boulant S. Cell Rep.

 

Interferons and viruses induce a novel truncated ACE2 isoform and not the full-length SARS-CoV-2 receptor. Onabajo OO, Banday AR, Stanifer ML, Yan W, Obajemu A, Santer DM, Florez-Vargas O, Piontkivska H, Vargas JM, Ring TJ, Kee C, Doldan P, Tyrrell DL, Mendoza JL, Boulant S, Prokunina-Olsson L. Nat Genet.

 

Development of Feline Ileum- and Colon-Derived Organoids and Their Potential Use to Support Feline Coronavirus Infection. Tekes G, Ehmann R, Boulant S, Stanifer ML. Cells.

 

The origin of diarrhea in rotavirus infection. Stanifer M, Boulant S. Science.

 

3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway. Kounatidis I, Stanifer ML, Phillips MA, Paul-Gilloteaux P, Heiligenstein X, Wang H, Okolo CA, Fish TM, Spink MC, Stuart DI, Davis I, Boulant S, Grimes JM, Dobbie IM, Harkiolaki M. Cell.

 

A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples. Dao Thi VL, Herbst K, Boerner K, Meurer M, Kremer LP, Kirrmaier D, Freistaedter A, Papagiannidis D, Galmozzi C, Stanifer ML, Boulant S, Klein S, Chlanda P, Khalid D, Barreto Miranda I, Schnitzler P, Kräusslich HG, Knop M, Anders S. Sci Transl Med.

 

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies. Cortese M, Lee JY, Cerikan B, Neufeldt CJ, Oorschot VMJ, Köhrer S, Hennies J, Schieber NL, Ronchi P, Mizzon G, Romero-Brey I, Santarella-Mellwig R, Schorb M, Boermel M, Mocaer K, Beckwith MS, Templin RM, Gross V, Pape C, Tischer C, Frankish J, Horvat NK, Laketa V, Stanifer M, Boulant S, Ruggieri A, Chatel-Chaix L, Schwab Y, Bartenschlager R. Cell Host Microbe.

 

SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography. Klein S, Cortese M, Winter SL, Wachsmuth-Melm M, Neufeldt CJ, Cerikan B, Stanifer ML, Boulant S, Bartenschlager R, Chlanda P. Nat Commun.

 

Forces during cellular uptake of viruses and nanoparticles at the ventral side. Wiegand T, Fratini M, Frey F, Yserentant K, Liu Y, Weber E, Galior K, Ohmes J, Braun F, Herten DP, Boulant S, Schwarz US, Salaita K, Cavalcanti-Adam EA, Spatz JP. Nat Commun.

 

NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice. Léger P, Nachman E, Richter K, Tamietti C, Koch J, Burk R, Kummer S, Xin Q, Stanifer M, Bouloy M, Boulant S, Kräusslich HG, Montagutelli X, Flamand M, Nussbaum-Krammer C, Lozach PY. Nat Commun.

 

Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses. Woelfl F, Léger P, Oreshkova N, Pahmeier F, Windhaber S, Koch J, Stanifer M, Roman Sosa G, Uckeley ZM, Rey FA, Boulant S, Kortekaas J, Wichgers Schreur PJ, Lozach PY. Viruses.

 

 

2019

Hypoxic Environment Promotes Barrier Formation in Human Intestinal Epithelial Cells through Regulation of MicroRNA 320a Expression. Muenchau S, Deutsch R, de Castro IJ, Hielscher T, Heber N, Niesler B, Lusic M, Stanifer ML, Boulant S. Mol Cell Biol.

 

Differential Regulation of Type I and Type III Interferon Signaling. Stanifer ML, Pervolaraki K, Boulant S. Int J Mol Sci.

 

Type-Specific Crosstalk Modulates Interferon Signaling in Intestinal Epithelial Cells. Pervolaraki K, Guo C, Albrecht D, Boulant S, Stanifer ML. J Interferon Cytokine Res.

 

Teratogenic Rubella Virus Alters the Endodermal Differentiation Capacity of Human Induced Pluripotent Stem Cells. Bilz NC, Willscher E, Binder H, Böhnke J, Stanifer ML, Hübner D, Boulant S, Liebert UG, Claus C. Cells.

 

Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses. Fakhiri J, Schneider MA, Puschhof J, Stanifer M, Schildgen V, Holderbach S, Voss Y, El Andari J, Schildgen O, Boulant S, Meister M, Clevers H, Yan Z, Qiu J, Grimm D. Mol Ther Methods Clin Dev.

 

TRIM69 Inhibits Vesicular Stomatitis Indiana Virus. Rihn SJ, Aziz MA, Stewart DG, Hughes J, Turnbull ML, Varela M, Sugrue E, Herd CS, Stanifer M, Sinkins SP, Palmarini M, Wilson SJ. J Virol.

 

2018

Clathrin-adaptor ratio and membrane tension regulate the flat-to-curved transition of the clathrin coat during endocytosis. Bucher D, Frey F, Sochacki KA, Kummer S, Bergeest JP, Godinez WJ, Kräusslich HG, Rohr K, Taraska JW, Schwarz US, Boulant S. Nat Commun.

 

Differential induction of interferon stimulated genes between type I and type III interferons is independent of interferon receptor abundance. Pervolaraki K, Rastgou Talemi S, Albrecht D, Bormann F, Bamford C, Mendoza JL, Garcia KC, McLauchlan J, Höfer T, Stanifer ML, Boulant S. PLoS Pathog.

 

Assaying the Contribution of Membrane Tension to Clathrin-Mediated Endocytosis.

Boulant S. Methods Mol Biol.

 

Reversible Fusion Proteins as a Tool to Enhance Uptake of Virus-Functionalized LbL Microcarriers. Scheffler K, Claus C, Stanifer ML, Boulant S, Reibetanz U. Biomacromolecules.

 

A PRDX1-p38α heterodimer amplifies MET-driven invasion of IDH-wildtype and IDH-mutant gliomas. Wirthschaft P, Bode J, Simon AEM, Hoffmann E, van Laack R, Krüwel T, Dietrich F, Bucher D, Hahn A, Sahm F, Breckwoldt MO, Kurz FT, Hielscher T, Fischer B, Dross N, Ruiz de Almodovar C, von Deimling A, Herold-Mende C, Plass C, Boulant S, Wiestler B, Reifenberger G, Lichter P, Wick W, Tews B. Int J Cancer. 2018

 

Surface Immobilization of Viruses and Nanoparticles Elucidates Early Events in Clathrin-Mediated Endocytosis. Fratini M, Wiegand T, Funaya C, Jiang Z, Shah PNM, Spatz JP, Cavalcanti-Adam EA, Boulant S. ACS Infect Dis.

 

Rubella Virus Strain-Associated Differences in the Induction of Oxidative Stress Are Independent of Their Interferon Activation. Zobel S, Lorenz M, Frascaroli G, Böhnke J, Bilz NC, Stanifer ML, Boulant S, Bergs S, Liebert UG, Claus C. Viruses.

 

2017

Reovirus inhibits interferon production by sequestering IRF3 into viral factories. Stanifer ML, Kischnick C, Rippert A, Albrecht D, Boulant S. Sci Rep.

 

Genome packaging of reovirus is mediated by the scaffolding property of the microtubule network. Shah PNM, Stanifer ML, Höhn K, Engel U, Haselmann U, Bartenschlager R, Kräusslich HG, Krijnse-Locker J, Boulant S. Cell Microbiol.

 

Type I and Type III Interferons Display Different Dependency on Mitogen-Activated Protein Kinases to Mount an Antiviral State in the Human Gut.Pervolaraki K, Stanifer ML, Münchau S, Renn LA, Albrecht D, Kurzhals S, Senís E, Grimm D, Schröder-Braunstein J, Rabin RL, Boulant S. Front Immunol.

 

miR-16 and miR-125b are involved in barrier function dysregulation through the modulation of claudin-2 and cingulin expression in the jejunum in IBS with diarrhoea. Martínez C, Rodiño-Janeiro BK, Lobo B, Stanifer ML, Klaus B, Granzow M, González-Castro AM, Salvo-Romero E, Alonso-Cotoner C, Pigrau M, Roeth R, Rappold G, Huber W, González-Silos R, Lorenzo J, de Torres I, Azpiroz F, Boulant S, Vicario M, Niesler B, Santos J. Gut.

 

Bin1 directly remodels actin dynamics through its BAR domain. Dräger NM, Nachman E, Winterhoff M, Brühmann S, Shah P, Katsinelos T, Boulant S, Teleman AA, Faix J, Jahn TR. EMBO Rep.

 

Mechanism of membrane fusion induced by vesicular stomatitis virus G protein. Kim IS, Jenni S, Stanifer ML, Roth E, Whelan SP, van Oijen AM, Harrison SC. Proc Natl Acad Sci U S A.

 

2016

Reovirus intermediate subviral particles constitute a strategy to infect intestinal epithelial cells by exploiting TGF-β dependent pro-survival signaling. Stanifer ML, Rippert A, Kazakov A, Willemsen J, Bucher D, Bender S, Bartenschlager R, Binder M, Boulant S. Cell Microbiol.

 

HIV-1 Vpu Antagonizes CD317/Tetherin by Adaptor Protein-1-Mediated Exclusion from Virus Assembly Sites. Pujol FM, Laketa V, Schmidt F, Mukenhirn M, Müller B, Boulant S, Grimm D, Keppler OT, Fackler OT. J Virol.

 

 

2015

 

Dynamics of virus-receptor interactions in virus binding, signaling, and endocytosis.

Boulant S, Stanifer M, Lozach PY. Viruses.

 

 

2014

 

Diverse intracellular pathogens activate type III interferon expression from peroxisomes. Odendall C, Dixit E, Stavru F, Bierne H, Franz KM, Durbin AF, Boulant S, Gehrke L, Cossart P, Kagan JC. Nat Immunol.

 

2013

Similar uptake but different trafficking and escape routes of reovirus virions and infectious subvirion particles imaged in polarized Madin-Darby canine kidney cells. Boulant S, Stanifer M, Kural C, Cureton DK, Massol R, Nibert ML, Kirchhausen T. Mol Biol Cell.

 

Arbidol inhibits viral entry by interfering with clathrin-dependent trafficking. Blaising J, Lévy PL, Polyak SJ, Stanifer M, Boulant S, Pécheur EI. Antiviral Res.

 

Silibinin inhibits hepatitis C virus entry into hepatocytes by hindering clathrin-dependent trafficking. Blaising J, Lévy PL, Gondeau C, Phelip C, Varbanov M, Teissier E, Ruggiero F, Polyak SJ, Oberlies NH, Ivanovic T, Boulant S, Pécheur EI. Cell Microbiol.

 

 

2012

Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers. Kural C, Tacheva-Grigorova SK, Boulant S, Cocucci E, Baust T, Duarte D, Kirchhausen T. Cell Rep.

 

The first five seconds in the life of a clathrin-coated pit. Cocucci E, Aguet F, Boulant S, Kirchhausen T. Cell.

 

Host cell nucleolin is required to maintain the architecture of human cytomegalovirus replication compartments. Strang BL, Boulant S, Kirchhausen T, Coen DM. mBio.

 

Human cytomegalovirus UL44 concentrates at the periphery of replication compartments, the site of viral DNA synthesis. Strang BL, Boulant S, Chang L, Knipe DM, Kirchhausen T, Coen DM. J Virol.

 

 

2011

 

Actin dynamics counteract membrane tension during clathrin-mediated endocytosis.

Boulant S, Kural C, Zeeh JC, Ubelmann F, Kirchhausen T. Nat Cell Biol.

 

Recruitment of cellular clathrin to viral factories and disruption of clathrin-dependent trafficking. Ivanovic T, Boulant S, Ehrlich M, Demidenko AA, Arnold MM, Kirchhausen T, Nibert ML. Traffic.

 

Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells. Thiery J, Keefe D, Boulant S, Boucrot E, Walch M, Martinvalet D, Goping IS, Bleackley RC, Kirchhausen T, Lieberman J. Nat Immunol.

 

 

2010

 

Peroxisomes are signaling platforms for antiviral innate immunity. Dixit E, Boulant S, Zhang Y, Lee AS, Odendall C, Shum B, Hacohen N, Chen ZJ, Whelan SP, Fransen M, Nibert ML, Superti-Furga G, Kagan JC. Cell.

 

Lipid metabolism and HCV infection. Targett-Adams P, Boulant S, Douglas MW, McLauchlan J. Viruses.

 

Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication. Strang BL, Boulant S, Coen DM. J Virol.

 

Requirement of cellular DDX3 for hepatitis C virus replication is unrelated to its interaction with the viral core protein. Angus AG, Dalrymple D, Boulant S, McGivern DR, Clayton RF, Scott MJ, Adair R, Graham S, Owsianka AM, Targett-Adams P, Li K, Wakita T, McLauchlan J, Lemon SM, Patel AH. J Gen Virol.

 

 

2009

Initiation of hepatitis C virus infection requires the dynamic microtubule network: role of the viral nucleocapsid protein. Roohvand F, Maillard P, Lavergne JP, Boulant S, Walic M, Andréo U, Goueslain L, Helle F, Mallet A, McLauchlan J, Budkowska A. J Biol Chem.

 

Subcellular localizations of the hepatitis C virus alternate reading frame proteins. Ratinier M, Boulant S, Crussard S, McLauchlan J, Lavergne JP. Virus Res.

 

 

2008

 

Hepatitis C virus core protein induces lipid droplet redistribution in a microtubule- and dynein-dependent manner. Boulant S, Douglas MW, Moody L, Budkowska A, Targett-Adams P, McLauchlan J. Traffic.

 

Visualization of double-stranded RNA in cells supporting hepatitis C virus RNA replication.Targett-Adams P, Boulant S, McLauchlan J. J Virol.

 

Maturation of hepatitis C virus core protein by signal peptide peptidase is required for virus production. Targett-Adams P, Hope G, Boulant S, McLauchlan J. J Biol Chem.

 

Transcriptional slippage prompts recoding in alternate reading frames in the hepatitis C virus (HCV) core sequence from strain HCV-1. Ratinier M, Boulant S, Combet C, Targett-Adams P, McLauchlan J, Lavergne JP. J Gen Virol.

 

2007

Disrupting the association of hepatitis C virus core protein with lipid droplets correlates with a loss in production of infectious virus. Boulant S, Targett-Adams P, McLauchlan J. J Gen Virol.

 

Expression of the alternative reading frame protein of Hepatitis C virus induces cytokines involved in hepatic injuries. Fiorucci M, Boulant S, Fournillier A, Abraham JD, Lavergne JP, Paranhos-Baccala G, Inchauspé G, Bain C. J Gen Virol.

 

The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly. Shavinskaya A, Boulant S, Penin F, McLauchlan J, Bartenschlager R. J Biol Chem.

 

2006

Structural determinants that target the hepatitis C virus core protein to lipid droplets. Boulant S, Montserret R, Hope RG, Ratinier M, Targett-Adams P, Lavergne JP, Penin F, McLauchlan J. J Biol Chem.

2005

Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. Boulant S, Vanbelle C, Ebel C, Penin F, Lavergne JP. J Virol.

 

Hepatitis C virus core protein acts as a trans-modulating factor on internal translation initiation of the viral RNA. Boni S, Lavergne JP, Boulant S, Cahour A. J Biol Chem.

 

2004

The hepatitis C virus Core protein is a potent nucleic acid chaperone that directs dimerization of the viral (+) strand RNA in vitro. Cristofari G, Ivanyi-Nagy R, Gabus C, Boulant S, Lavergne JP, Penin F, Darlix JL. Nucleic Acids Res.

2003

Unusual multiple recoding events leading to alternative forms of hepatitis C virus core protein from genotype 1b. Boulant S, Becchi M, Penin F, Lavergne JP. J Biol Chem.